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  • Detection and Decay of Different Classes of Environmental RNA (eRNA) from Zebrafish (Danio rerio)

    Purpose: This technical note contributes to the growing body of knowledge about macroscopic eukaryotic environmental RNA (eRNA) by exploring detection and decay for several different zebrafish (Danio rerio) eRNAs in a mesocosm setting. The study addressed four basic hypotheses: (1) D. rerio would deposit detectable levels of eRNA into water, (2) different classes of eRNA would be detected, (3) different eRNA sequences (for example, loci) would degrade at different rates, and (4) abiotic and biotic factors would influence rates of degradation. For the last hypothesis, we tracked eRNA concentration decay under treatments with different water temperatures and levels of microbiological activity, two factors known to significantly influence environmental DNA (eDNA) decay (Barnes et al. 2014; Lance et al. 2017; Nielsen et al. 2007; Strickler et al. 2015).
  • Reservoir Sediment Deltas of the Southwestern United States: Challenges and Opportunities for Riparian Vegetation Management

    Purpose: This technical note summarizes methods used to identify 58 reservoir sediment deltas in the Southwestern United States and California, behind 47 different dams, with potential regional importance as breeding or migratory stopover habitat for three species of riparian vegetation–dependent birds listed under the Endangered Species Act[1]: least Bell’s vireo (LBVI) (Vireo bellii pusillus), southwest willow flycatcher (SWFL) (Empidonax traillii extimus), and yellow-billed cuckoo (YBCU) (Coccyzus americanus). Information on inflow streams, drainage area, and management authority are provided for each delta. This technical note recommends follow-up research on the temporal dynamics of vegetation colonization, growth, and mortality on these deltas to explore the potential value of these geographically widespread hydrogeomorphic features on arid-land rivers for the conservation of riparian vegetation–dependent birds.
  • Rectifying and Stabilizing Planet SkySat Video Collects for Bathymetric Inversions from Space

    Abstract: This Coastal and Hydraulics Engineering Technical Note (CHETN) presents the development of a workflow to process Planet SkySat videos collected from space at the US Army Engineer Research and Development Center (ERDC), Coastal and Hydraulics Laboratory (CHL), Field Research Facility (FRF), in Duck, North Carolina, to derive wave kinematics and perform bathymetric inversions. The document summarizes the nine 30–60 s* satellite video collections, demonstrates the accuracy of an automated rectification and stabilization workflow, and applies a new short-dwell version of a common inversion algorithm (cBathy) to demonstrate the utility of short-dwell videos from space providing an initial out-of-the-box assessment of errors for one of the collections, and recommends future avenues of research for improving bathymetric predictions.
  • Regeneration Dynamics of Bottomland Hardwood Sites Following Prolonged Growing-Season Inundation

    Abstract: The spring flood of the Mississippi River and backwater areas in 2019 resulted in large-scale flooding and was the longest-lasting flood event since the Great Flood of 1927. This flood event provided a rare opportunity to establish permanent plots in batture and backwater habitats to evaluate forest-stand dynamics following prolonged flooding. In this study, we evaluated postflooding conditions of forest overstory, midstory, and regeneration by establishing permanent plots at four locations subjected to varying amounts of flooding within the Mississippi River batture and the Yazoo–Mississippi Delta backwater region. Our results highlight oak regeneration success following the 2019 flood event as well as the utility and need to establish and monitor permanent plots to increase our understanding of floodplain forest dynamics in regions experiencing prolonged riverine flooding during the growing season.
  • A/E/C Computer-Aided Design (CAD) Standard: Release 6.2

    Abstract: The A/E/C Computer-Aided Design (CAD) Standard has been developed by the CAD/Building Information Modeling (BIM) Technology Center for Facilities, Infrastructure, and Environment to eliminate redundant CAD standardization efforts within DoD and the Federal Government. This manual is part of an initiative to develop a nonproprietary CAD standard that incorporates existing industry, national, and international standards and to develop data standards that address the entire life cycle of facilities within DoD. The material addressed in the A/E/C CAD Standard includes level/layer assignments, digital file naming, and standard symbology. The CAD/BIM Center’s primary goal is to develop a CAD standard that is generic enough to operate under various CAD software packages (such as Bentley’s MicroStation and Autodesk’s AutoCAD) while incorporating existing industry standards when possible. While this Standard encompasses many CAD concepts and practices, it is not intended to limit the capabilities of other advanced modeling software. Ultimately, a BIM / Civil Information Modeling standard will be developed to standardize the additional capabilities of other software.
  • Signal Power Distributions for Simulated Outdoor Sound Propagation in Varying Refractive Conditions

    Abstract: Probability distributions of acoustic signals propagating through the near-ground atmosphere are simulated by the parabolic equation method. The simulations involve propagation at four angles relative to the mean wind, with frequencies of 100, 200, 400, and 800 Hz. The environmental representation includes realistic atmospheric refractive profiles, turbulence, and ground interactions; cases are considered with and without parametric uncertainties in the wind velocity and surface heat flux. The simulated signals are found to span a broad range of scintillation indices, from near zero to exceeding ten. In the absence of uncertainties, the signal power (or intensity) is fit well by a two-parameter gamma distribution, regardless of the frequency and refractive conditions. When the uncertainties are included, three-parameter distributions, namely, the compound gamma or generalized gamma, are needed for a good fit to the simulation data. The compound gamma distribution appears preferable because its parameters have a straight forward interpretation related to the saturation and modulation of the signal by uncertainties.
  • Permafrost and Groundwater Characterization at the Proximity of the Landfill, Fort Wainwright, Alaska

    Abstract: This report summarizes a site investigation at the vicinity of the landfill, a discontinuous permafrost site, at Fort Wainwright, Alaska. The objective of this effort was to characterize the permafrost extent and groundwater flow at the study area, and to compare newly collected subsurface characteristics with historical datasets. The main tasks for this effort included lidar and remote sensing analyses, geophysical investigations, a tracer dye study, contaminant trend analysis, and installation of soil temperature sensors. Findings included changes in stream channels and watershed boundaries, and elevation losses (0.2 m to 1 m) east and northeast of the landfill. From frost probe measurements, we found that depths to permafrost were up to 1.5 m deeper in 2021 than in 2010 where the difference in depth ranged from 20% to more than 350%. Furthermore, we detected a reduction in lateral permafrost extent from geophysical datasets. The groundwater flow direction, as detected through the dye study, was south to southwest. Dye was detected up to 2,300 m from the injection point. Groundwater travel times, as calculated from the dye study, varied greatly. For upcoming historical comparisons, it is recommended that data collections are performed using similar methods as described in this study.
  • Evaluation of the Version 1 Advanced Tactical Awareness Kit–Expeditionary Radar (ATAK-ER V1) for Accuracy and Reliability in Surf-Zone Characterization in a Range of Environmental Conditions

    Abstract: This Coastal and Hydraulics Engineering Technical Note (CHETN) presents the evaluation of a rapidly deployable radar and associated software for characterizing surf-zone waves, currents, and bathymetries at the US Army Engineer Research and Development Center (ERDC), Coastal and Hydraulics Laboratory (CHL), Field Research Facility (FRF), in Duck, North Carolina. This project was conducted at the request of the US Marine Corps (USMC) Warfighting Laboratory. The Version 1 Advanced Tactical Awareness Kit–Radar Expeditionary (ATAK-ER V1) system was deployed 15 times between July and August 2023 to observe a range of wave, water level, and wind conditions that could each affect radar processing. Products from the system were then compared to the FRF’s continuously operating in situ instruments and monthly bathymetric surveys to quantify the accuracy and reliability of the output. A number of issues with the unit are identified, including potential error sources contributing to inaccuracies, but the black-box nature of the commercial off-the-shelf (COTS) unit prevents a confident understanding of why wave heights are underpredicted (by 65% on average), why bathymetries consistently have root-mean-square errors (RMSE) over 1 m with progressively greater errors with distance offshore, or why some collections are unable to generate all of the advertised products. This Version 1 COTS unit is not recommended for operational use at this time.
  • Qualitative Habitat Evaluation Index for Louisville Streams (QHEILS)

    Purpose: Urban stream restoration typically involves multiple objectives addressing different aspects of ecosystem integrity, such as habitat provision, geomorphic condition, watershed connectivity, water quality, and land-use change. Multiple stream assessment tools and models have been developed and applied to inform restoration prioritization, planning, and design. Here, we present the Qualitative Habitat Evaluation Index for Louisville Streams (QHEILS, pronounced “quails”), which was designed as an interdisciplinary assessment method for urban streams in the Louisville, Kentucky, metropolitan region. The model adapts a regional habitat assessment procedure, the Qualitative Habitat Evaluation Index (QHEI), by incorporating additional processes related to geomorphic change and watershed connectivity. The QHEILS was developed in the context of the Beargrass Creek Ecosystem Restoration Feasibility Study, and it provides a rapid procedure for assessing multiobjective benefits associated with proposed restoration actions. This technical note summarizes the model and provides example applications within the Beargrass Creek watershed.
  • Sharing Ships’ Weather Data via AIS: Concept and Results from Multiyear Observations

    Abstract: The purpose of this Coastal and Hydraulics Engineering technical note (CHETN) is to discuss the concept, demonstrations, and the initial results of multiyear proof-of-concept testing of the capability to share weather data from ships via the Automatic Identification System (AIS). Technical foundations of this process were described by Tetreault and Johnson (2020) with partial results described in Johnston et al. 2021. The updated results in this CHETN include evaluation of the efficacy of the various application-specific message (ASM) formats use to communicate the weather observations and data reception results for selected vessels that have been participating in the proof-of-concept field deployment since 2019 or later.