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Category: Publications: Environmental Laboratory (EL)
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  • Bioaugmentation for Enhanced Mitigation of Explosives in Surface Soil

    Abstract: Residual munition constituents (MCs) generated from live-fire training exercises persist in soil and can migrate to groundwater, surface waters, and off-range locations. Techniques to mitigate this potential migration are needed. Since the MC hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) can be biodegraded, soil inoculation with RDX-degrading bacteria (i.e., bioaugmentation) was investigated as a means to reduce the migration potential of RDX. Metagenomic studies using contaminated soils have suggested that a greater diversity of bacteria are capable of RDX biodegradation. However, these bacteria remain uncultivated and are potentially a source of novel enzymes and pathways for RDX biodegradation. In situ soil cultivation of a novel soil array was used to isolate the uncultivated bacteria that had been inferred to degrade RDX. Approximately 10.5% of the bacteria isolated from the soil arrays degraded RDX by the aerobic denitration pathway. Of these, 26.5% were possibly novel species of RDX-degrading bacteria, based on 16S rRNA sequence similarity. Both cell encapsulation in hydrogels and coating cells onto granules of polymeric carbon sources were investigated as carrier/delivery approaches for soil inoculation. However, neither of these approaches could confirm that the observed RDX degradation was by the inoculated bacteria.
  • Financing Natural Infrastructure: The Elizabeth River Project, Chesapeake Bay, VA

    Purpose: Knowledge gaps surrounding natural infrastructure (NI) life cycles and performance thwart widespread implementation of NI in civil works projects. In particular, information about funding or financing the scoping, design, construction, monitoring, and adaptive management of NI projects constitutes a key need as there is no standard process for securing funds. This technical note is part of a series documenting successful examples of funding NI projects and sharing lessons learned about a variety of funding and financing methods to increase the implementation of NI projects. The research effort is a collaboration between the Engineering With Nature® (EWN®) and Systems Approach to Geomorphic Engineering (SAGE) programs of the US Army Corps of Engineers (USACE). This technical note explores how the Elizabeth River Project (ERP), a nonprofit organization based in Norfolk, Virginia, developed a homeowner cost-sharing program to fund NI projects—living shorelines, rain gardens, and riparian buffers—within an urban watershed.
  • The Use of Rhodamine Water Tracer (RWT) Dye to Improve Submersed Herbicide Applications

    Abstract: The inert fluorescent dye rhodamine water tracer (RWT) has been widely used in freshwater aquatic systems for many years to quantify bulk water exchange patterns and as a tracer for submersed herbicide movement. The dye is well-suited for tracer work due to its high solubility and detectability in water (<0.01 μg/L). Federal guidelines limit the aqueous concentration 0f RWT to <10 μg/L at drinking water intakes. The dye has proven to be harmless to aquatic organisms and humans in low concentrations and is relatively inexpensive. Since 1991, RWT has been used by Engineer Re-search and Development Center (ERDC) researchers to simulate aqueous herbicide applications in large, hydrodynamic systems in over 12 states. Such simulations have improved the effectiveness of herbicide treatments by linking in situ water exchange processes with appropriate herbicide selection and application rates. Understanding these parameters can be critical for mitigating herbicide exposure in environmentally sensitive settings and around potable water and irrigation intakes. A data-based estimate of water exchange patterns usually results in successful submersed herbicide applications—both with target-plant efficacy and limited injury to nontarget vegetation. Using RWT dye to simulate submersed herbicide applications is an important predictive and real-time tool in both experimental and operational settings.
  • Embracing Biodiversity on Engineered Coastal Infrastructure through Structured Decision-Making and Engineering With Nature

    Abstract: Extreme weather variation, natural disasters, and anthropogenic actions negatively impact coastal communities through flooding and erosion. To safeguard coastal settlements, shorelines are frequently reinforced with seawalls and bulkheads. Hardened shorelines, however, result in biodiversity loss and environmental deterioration. The creation of sustainable solutions that engineer with nature is required to lessen natural and anthropogenic pressures. Nature-based solutions (NbS) are a means to enhance biodiversity and improve the environment while meeting engineering goals. To address this urgent need, the US Army Corps of Engineers (USACE) Engineering With Nature® (EWN) program balances economic, environmental, and social benefits through collaboration. This report presents how design and engineering practice can be enhanced through organized decision-making and landscape architectural renderings that integrate engineering, science, and NbS to increase biodiversity in coastal marine habitats. When developing new infrastructure or updating or repairing existing infrastructure, such integration can be greatly beneficial. Further, drawings and renderings exhibiting EWN concepts can assist in decision-making by aiding in the communication of NbS designs. Our practical experiences with the application of EWN have shown that involving landscape architects can play a critical role in effective collaboration and result in solutions that safeguard coastal communities while maintaining or enhancing biodiversity.
  • Cut and Capture System Technology for Demilitarization of Underwater Munitions

    Abstract: Munitions are encountered in a variety of underwater environments as unexploded ordnance (UXO) or munitions and explosives of concern (MEC). These items can cause unacceptable explosive risks to critical infrastructure, recreational divers, and fishermen. The primary goal of the demonstrations was to validate an underwater suite of tools that can be used to render underwater UXO and MEC safe in shallow water (i.e., up to 100 ft). US Navy underwater ranges in the Gulf of Mexico, south of the Naval Support Activity–Panama City, were selected for the first two demonstrations to fully display the integrated system by processing inert munitions, such as the Navy 5 in./38 cal and the Army 105 mm High Explosive (HE) M1 projectile. The third demonstration, however, occurred at the Naval Surface Warfare Center (NSWC), Crane, Lake Glendora Test Facility, in Sullivan, Indiana. Twenty US Army 105 mm HE M1 projectiles filled with TNT were successfully processed. Overall, this project showed that Gradient Technology’s high-pressure waterjet demilitarization technology can be reliably operated underwater at depths less than 100 ft of seawater when the supporting equipment is located on the deck of a vessel or floating pier system.
  • Deployable Resilient Installation Water Purification and Treatment System (DRIPS): Geoenabled Water Production and Disinfection Systems for Installations

    Abstract: The Deployable Resilient Installation water Purification and treatment System (DRIPS) was delivered to aid an Organic Industrial Base in increasing their Installation Status Report–Mission Capacity (ISR-MC) score from black to green as part of a Course of Action (COA) within their Installation Energy and Water Plan (IEWP). DRIPS was also intended to help them be better prepared for the future in meeting their water and energy requirement goals for sustainment of critical missions. The IEWP ISR-MC requirements were met upon implementation of this project. Overall, the purpose of the DRIPS is to be a critical asset in disaster response and military operations, providing a reliable and effective means of producing potable water and disinfection in challenging and unpredictable environments. Its adaptability, mobility, and comprehensive water treatment capabilities make it an invaluable resource for addressing water-related emergencies and water disruptions and for sustaining critical missions. It also addresses a point of need by improving the ability to meet demands, reducing convoy requirements and the logistical footprint, facilitating the endurance of expeditionary forces, and ensuring the well-being of affected installations during times of disaster response, training operations, normal water disruptions, and emergency preparation.
  • Evaluation of an Operational Demonstration of a Potential Aquatic Plant Control and Nutrient Mitigation Technology in Lake Okeechobee, Florida

    Purpose: This technical note describes the findings from a field-demonstration project conducted in 2022 that aimed to mechanically harvest water hyacinth (Eichhornia crassipes (Mart.) Solms) in Lake Okeechobee, Florida; macerate the harvested plant material; and pump it as a slurry to a terrestrial disposal site as a potential novel technology to reduce total phosphorus (TP) in the system.
  • Employing Ultrafiltration and Reverse Osmosis (UF/RO) for Treatment of Source-Separated Graywater: ER-201636

    Abstract: In field operating environments, military units must ensure access to a critical water supply to maintain mission readiness. Increasing complexity of logistics and costs to transport water and climate change are driving the development and demonstration of water treatment units. The treatment unit described uses ultrafiltration (UF) and reverse osmosis (RO) membrane technologies with a disinfection step to treat low-strength graywater from shower facilities at Camp Shelby Joint Force Training Center in Hattiesburg, Mississippi. Samples were collected from human shower sampling events. During the demonstration, greater than 100,000 gal. of graywater were treated, supplied by two battalion training rotations over a 6-month period. Characterization of the source graywater continued throughout the system demonstration. Based on the literature, these are among the largest source-separated graywater sampling events to date. The combined treatment train of UF/RO met all compliance metrics for all analytes of interest for potability and met nonpotable metrics except for special use cases in three states. Both the quality of the treated water and the effective volume gains from an 85% multiplier effect directly support DoD water sustainment goals for both installations and operational environments.
  • Approach for On-Site, On-Demand Contaminant-Removal Devices Enabled by Low-Cost 3D Printing

    Purpose: The purpose of this technical note is to disseminate methods to design and create a 3D device that could be used to determine relative toxicity potential of existing and emerging contaminants of concern in situ for sediment shoaled in federal navigation channels prior to being dredged. This device has the potential to reduce the cost of conventional sediment evaluations conducted prior to dredging operations.
  • Risk-Based Prioritization of Operational Condition Assessments: Trinity River and Willamette River Case Studies

    Abstract: The US Army Corps of Engineers (USACE) operates, maintains, and man-ages over 700 dams and 4,000 miles of levees, providing approximately $257 billion worth of economic benefit to the Nation. USACE employs the Operational Condition Assessment (OCA) process to understand the condition of those assets and allocate resources to minimize risk associated with performance degradation. Understanding risk in flood risk management (FRM) assets requires an understanding of consequence of asset failure from a systemwide FRM watershed perspective and an understanding of likelihood of degradation based on the condition of the low-level components derived from OCA ratings. This research demonstrates a case-study application of a scalable methodology to model the likelihood of a dam performing as expected given the state of its gates and their components. The research team combines this likelihood of degradation with consequences generated by the application of designed simulation experiments with hydrological models to develop risk measures. These risk measures can be developed for all FRM gate assets in order to enable traceable, consistent resource allocation decisions. Two case study applications are provided.