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Archive: August, 2021
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  • Geographic and Genetic Variation in Susceptibility of Butomus umbellatus to Foliar Fungal Pathogens

    Abstract: Large-scale patterns of plant invasions may reflect regional heterogeneity in biotic and abiotic factors and genetic variation within and between invading populations. Having information on how effects of biotic resistance vary spatially can be especially important when implementing biological control because introduced agents may have different Impacts through interactions with host-plant genotype, local environment, or other novel enemies. We conducted a series of field surveys and laboratory studies to determine whether there was evidence of biotic resistance, as foliar fungal pathogens, in two introduced genotypes (triploid G1, diploid G4) of the Eurasian wetland weed, Butomus umbellatus L. in the USA. We tested whether genotypes differed in disease attack and whether spatial patterns in disease incidence were related to geographic location or climate for either genotype. After accounting for location (latitude, climate), G1 plants had lower disease incidence than G4 plants in the field (38% vs. 70%) but similar pathogen richness. In contrast, bioassays revealed G1 plants consistently received a higher damage score and had larger leaf lesions regardless of pathogen. These results demonstrate that two widespread B. umbellatus genotypes exhibit different susceptibility to pathogens and effectiveness of pathogen biological controls may depend on local conditions.
  • Crevice Corrosion and Environmentally Assisted Cracking of High-Strength Duplex Stainless Steels in Simulated Concrete Pore Solutions

    Abstract: This paper presents a study of crevice corrosion and environmentally assisted cracking (EAC) mechanisms in UNS S32205 and S32304 which were cold drawn to tensile strengths of approximately 1300 MPa. The study utilized a combination of electrochemical methods and slow strain rate testing to evaluate EAC susceptibility. UNS S32205 was not susceptible to crevice corrosion in stranded geometries at Cl- concentrations up to 1.0 M in alkaline and carbonated simulated concrete pore solutions. UNS S32304 did exhibit a reduction in corrosion resistance when tested in a stranded geometry. UNS S32205 and S32304 were not susceptible to stress corrosion cracking at Cl- concentrations up to 0.5 M in alkaline and carbonated solutions but were susceptible to hydrogen embrittlement with cathodic overprotection.
  • Terrain Coefficients for Predicting Energy Costs of Walking over Snow

    Abstract: Predicting the energy costs of human travel over snow can be of significant value to the military and other agencies planning work efforts when snow is present. The ability to quantify, and predict, those costs can help planners determine if snow will be a factor in the execution of dismounted tasks and operations. To adjust predictive models for the effect of terrain, and more specifically for surface conditions, on energy costs, terrain coefficients (ƞ) have been developed. By applying knowledge gained from prior studies of the effects of terrain and snow, and by leveraging those existing dismounted locomotion models, we seek to outline the steps in developing an improved terrain coefficient (ƞ) for snow to be used in predictive modeling. Using published data, methods, and a well-informed understanding of the physical elements of terrain, e.g., characterization of snow sinkage (z), this study made adjustments to ƞ-values specific to snow. This review of published metabolic cost methods suggest that an improved ƞ-value could be developed for use with the Pandolf equation, where z=depth (h)*(1 - (snow density (ρ0)/1.186)) and ƞ=0.0005z3 + 0.0001z2 + 0.1072z + 1.2604. This paper provides data-driven improvements to models that are used to predict the energy costs of dismounted movements over snow.
  • Houston Ship Channel Expansion Channel Improvement Project (ECIP) Numerical Modeling Report: BABUS Cell and Bird Island Analysis

    Abstract: The Houston Ship Channel (HSC) is one of the busiest deep-draft navigation channels in the United States and must be able to accommodate increasing vessel sizes. The US Army Engineer District, Galveston (SWG), requested the Engineer Research and Development Center, Coastal and Hydraulics Laboratory, perform hydrodynamic and sediment modeling of proposed modifications in Galveston and Trinity Bays and along the HSC. The modeling results are necessary to provide data for hydrodynamic, salinity, and sediment transport analysis. SWG provided three project alternatives that include closing Rollover Pass, Bay Aquatic Beneficial Use System cells, Bird Islands, and HSC modifications. These alternatives and a Base (existing condition) will be simulated for present (2029) and future (2079) conditions. The results of these alternatives/conditions as compared to the Base are presented in this report. The model shows that the mean salinity varies by 2–3 ppt due to the HSC channel modifications and by approximately 5 ppt in the area of East Bay due to the closure of Rollover Pass. The tidal prism increases by 2.5% to 5% in the alternatives. The tidal amplitudes change by less than 0.01 m. The residual velocity vectors vary in and around areas where project modifications are made.
  • The Response of Vegetated Dunes to Wave Attack

    Abstract: Vegetation is believed to increase the stability of dunes during wave attack, but limited data is available. A physical model study was performed to evaluate changes in the dune stability with and without biomass, both above and belowground. The above and belowground biomass was modeled using wooden dowels and coir fibers, respectively. For both the collision and overwash storm impact regimes, the results of this study clearly demonstrate that the inclusion of biomass in the model dune reduces the erosion and overwash. The combination of both above and belowground biomass was the most effective at reducing erosion followed by belowground biomass, with aboveground biomass providing the smallest benefit regardless of the wave condition and water level. Additionally, the overwash of sediment and water was decreased with the inclusion of biomass, following the same trends as the erosion. As the dune eroded, the storm impact regime transitioned from collision to overwash. The inclusion of biomass delays this transition in storm impact regime, providing greater protection to coastal communities. This study highlights the need to consider dune vegetation for dune construction and coastal planning.
  • The AFWA Dust Emission Scheme for the GOCART Aerosol Model in WRF-Chem v3.8.1

    Abstract: Airborne particles of mineral dust play a key role in Earth’s climate system and affect human activities around the globe. The numerical weather modeling community has undertaken considerable efforts to accurately forecast these dust emissions. Here, for the first time in the literature, we thoroughly describe and document the Air Force Weather Agency (AFWA) dust emission scheme for the Georgia Institute of Technology–Goddard Global Ozone Chemistry Aerosol Radiation and Transport (GOCART) aerosol model within the Weather Research and Forecasting model with chemistry (WRF-Chem) and compare it to the other dust emission schemes available in WRF-Chem. The AFWA dust emission scheme addresses some shortcomings experienced by the earlier GOCART-WRF scheme. Improved model physics are designed to better handle emission of fine dust particles by representing saltation bombardment. WRF-Chem model performance with the AFWA scheme is evaluated against observations of dust emission in southwest Asia and compared to emissions predicted by the other schemes built into the WRF-Chem GOCART model. Results highlight the relative strengths of the available schemes, indicate the reasons for disagreement, and demonstrate the need for improved soil source data.
  • Expression and Secretion of Active Moringa oleifera Coagulant Protein in Bacillus subtilis

    Abstract: Cationic polypeptide proteins found in the seeds of the tropical plant Moringa oleifera have coagulation efficiencies similar to aluminum and ferric sulfates without their recalcitrant nature. Although these proteins possess great potential to augment or replace traditional coagulants in water treatment, harvesting active protein from seeds is laborious and not cost-effective. Here, we describe an alternative method to express and secrete active M. oleifera coagulant protein (MO) in Bacillus subtilis. A plasmid library containing the MO gene and 173 different types of secretory signal peptides was created and cloned into B. subtilis strain RIK1285. Fourteen of 440 clones screened were capable of secreting MO with yields ranging from 55 to 122 mg/L of growth medium. The coagulant activity of the highest MO secreting clone was evaluated when grown on Luria broth, and cell-free medium from the culture was shown to reduce turbidity in a buffered kaolin suspension by approximately 90% compared with controls without the MO gene. The clone was also capable of secreting active MO when grown on a defined synthetic wastewater supplemented with 0.5% tryptone. Cell-free medium from the strain harboring the MO gene demonstrated more than a 2-fold reduction in turbidity compared with controls. Additionally, no significant amount of MO was observed without the addition of the synthetic wastewater, suggesting that it served as a source of nutrients for the effective expression and translocation of MO into the medium.
  • Reproducibility Assessment and Uncertainty Quantification in Subjective Dust Source Mapping

    Abstract: Accurate dust-source characterizations are critical for effectively modeling dust storms. A previous study developed an approach to manually map dust plume-head point sources in a geographic information system (GIS) framework using Moderate Resolution Imaging Spectroradiometer (MODIS) imagery processed through dust-enhancement algorithms. With this technique, the location of a dust source is digitized and recorded if an analyst observes an unobscured plume head in the imagery. Because airborne dust must be sufficiently elevated for overland dust-enhancement algorithms to work, this technique may include up to 10 km in digitized dust-source location error due to downwind advection. However, the potential for error in this method due to analyst subjectivity has never been formally quantified. In this study, we evaluate a version of the methodology adapted to better enable reproducibility assessments amongst multiple analysts to determine the role of analyst subjectivity on recorded dust source location error. Four analysts individually mapped dust plumes in Southwest Asia and Northwest Africa using five years of MODIS imagery collected from 15 May to 31 August. A plume-source location is considered reproducible if the maximum distance between the analyst point-source markers for a single plume is ≤10 km. Results suggest analyst marker placement is reproducible; however, additional analyst subjectivity-induced error (7 km determined in this study) should be considered to fully characterize locational uncertainty. Additionally, most of the identified plume heads (> 90%) were not marked by all participating analysts, which indicates dust source maps generated using this technique may differ substantially between users.
  • Geotechnical Inspection and Technical Review of Santa Margarita River Marine Corps Air Station Levee, U.S. Marine Corps, Camp Pendleton, CA, 19-20 November 2019

    Abstract: This report describes activities performed, results obtained, and conclusions made from an independent technical review of past levee inspections and the proposed remediation plan for the Santa Margarita Levee that surrounds the U.S. Marine Corps Air Station (MCAS) Camp Pendleton. In support of the technical review, ERDC personnel performed a supplemental levee inspection on 19 and 20 November 2019 with MCAS personnel. Previous levee inspections had rated the levee system as Unacceptable due to unwanted vegetation encroaching on the levee right-of-way, which prevents full inspection during flooding. Concerns were raised by the U.S. Fish and Wildlife (USFW) about environmental impacts of the proposed remediation measures and the necessity of such actions. USFW personnel requested an engineering review from an independent party, and ERDC was tasked with performing the independent technical review. The following special report describes the tasks performed and results obtained from the independent technical review.
  • Trace Explosives Detection by Cavity Ring-Down Spectroscopy (CRDS)

    Abstract: We built three successive versions of a thermal decomposition cavity ring-down spectrometer and tested their response to explosives. These explosive compound analyzers successfully detected nitroglycerine, 2,4,6-trinitrotoluene (TNT), pentaerythryl tetranitrate, hexahydro-1,3,5-trinitro-s-triazine and triacetone triperoxide (TATP). We determined the pathlength and limits of detection for each, with the best limit of detection being 13 parts per trillion (ppt) of TNT. For most of the explosive tests, the peak height was higher than the expected value, meaning that peroxy radical chain propagation was occurring with each of the explosives and not just the peroxide TATP.