12 Feb 2026

Growth Patterns of Three Hydrilla Verticillata Biotypes in the United States

Abstract: Hydrilla [Hydrilla verticillata (L.f. Royle)] is an invasive submersed plant first documented in the United States (US) in the 1950s. Until recently, only two distinct strains of hydrilla, hereafter referred to as biotypes, were recorded in the US with both belonging to the same clade and characterized as either ‘monoecious’ or ‘dioecious’. In 2016, a third genetically distinct biotype (‘clade C’) was identified in the lower Connecticut River, Connecticut. Trials were conducted at two geographically unique locations within North Carolina to compare growth and reproduction of hydrilla biotypes by climatic conditions. Nominal differences were observed in peak aboveground biomass, relative growth rate, or occurrence of these metrics among the three hydrilla biotypes, although the predicted peak biomass for dioecious hydrilla did not occur during the study period. Monoecious hydrilla exhibited lower peak belowground biomass than dioecious and clade C hydrilla despite its abundant subterranean turion production. Monoecious hydrilla exhibited 1.8X greater aboveground biomass 180 days after planting (Julian day 304) than clade C hydrilla. Clade C hydrilla produced 74 axillary turions per plant on Julian day 304, which equates to a 2.3X increase over monoecious hydrilla and 61.5X increase over dioecious hydrilla. Conversely, subterranean turion production was 4.7 and 7.1X higher in monoecious hydrilla and dioecious hydrilla than clade C hydrilla, respectively. These studies suggest the clade C hydrilla displays many phenological similarities to the other two biotypes in the US, but its prodigious axillary turion production poses concerning implications for potential to spread and persistence following management.

31 May 2023

Small Plot Applications of Florpyrauxifen –Benzyl (Procellacor SC™) for Control of Monoecious Hydrilla in Roanoke Rapids Lake, NC

Abstract: Four demonstration plots were selected at Roanoke Rapids Lake, NC to evaluate water exchange and aqueous herbicide residues in stands of submersed aquatic vegetation (SAV) following treatment with rhodamine wt dye and florpyrauxifen-benzyl to control monecious hydrilla. Florpyrauxifen-benzyl (Procellacor™ SC) was applied in combination with Rhodamine WT (RWT) at two of the plots. Dye measurements and herbicide residue samples were collected at specific time intervals to draw comparisons between herbicide and RWT dye dissipation. The two additional plots served as reference plots to the treatment plots. Pre- and post-treatment vegetation surveys were conducted to evaluate monoecious hydrilla control and non-target species response. RWT dye and herbicide residue data indicated rapid water exchange was occurring with each treatment plot. As a result, florpyrauxifen-benzyl concentration and exposure times (CETs) towards monoecious hydrilla were not sufficient to achieve adequate control by 4 weeks after treatment (WAT). To reduce the impact of hydraulic complexity and improve herbicide efficacy, treatments should coincide with minimal reservoir discharge events to extend herbicide CET relationships. Evaluations of florpyrauxifen-benzyl on late season, mature plants may have impacted herbicide efficacy. Evaluations should be conducted earlier in the growing season, on young, actively growing plants, to discern potential differences in efficacy due to treatment timing and phenology. More information on herbicide concentration and exposure time relationships for monoecious hydrilla should be developed in growth chamber and mesocosm settings to improve species selective management of monoecious hydrilla in hydrodynamic reservoirs.

23 Sep 2021

Efficacy of Florpyrauxifen-benzyl on Dioecious Hydrilla and Hybrid Water Milfoil - Concentration and Exposure Time Requirements

Abstract: This study conducted small-scale trials under various concentration and exposure time (CET) scenarios to determine florpyrauxifen-benzyl activity on dioecious hydrilla and hybrid watermilfoil and determine impact on water stargrass and elodea. Hydrilla treated with 12, 24, or 36 μg active ingredient (a.i.) L⁻¹ florpyrauxifen-benzyl and exposed for 12, 24, or 48 hr under outdoor mesocosm conditions was reduced in biomass by 30-75% at 8 weeks after treatment (WAT). An additional hydrilla trial at the same herbicide concentrations, but under longer exposures (24, 72, or 168 hr), resulted in 33–85% plant control. Under indoor conditions, hybrid watermilfoil dry weight decreased 98–100% with subsurface applications of florpyrauxifen-benzyl under CET scenarios of 3–12 μg a.i. L⁻¹ at 3–24 hr exposure times in a growth chamber trial. Under shorter exposure periods (0.5–4 hr) in a follow-up trial, low doses (3–9 μg a.i. L⁻¹) achieved 50–100% control of hybrid watermilfoil. In the same trial, the nontarget species water stargrass and elodea proved relatively tolerant to the florpyrauxifen-benzyl at doses up to 6 μg a.i. L⁻¹ (4 hr exposure) and 9 μg a.i. L⁻¹ (1 hr exposure). These small-scale trials demonstrate florpyrauxifen-benzyl’s potential to selectively manage invasive species.