29 Oct 2025

A Multigenerational Exposure of the Zebrafish (Danio rerio) to PFOS

Abstract: The ecological risk of PFOS in extended chronic and multigenerational exposures was quantified through survival, growth, reproduction, and vitellogenin (egg yolk protein precursor) responses as well as PFOS bioconcentration in zebrafish (Danio rerio). Fish were exposed to environmentally relevant PFOS concentrations through 180 days postfertilization (dpf) in the parental (P) and first filial (F1) generations and 16 dpf in the second filial (F2) generation. Survival decreased significantly in P and F2 generation exposures, but not F1, at the highest PFOS treatment. Significant adverse effects on body weight and length occurred predominantly at highest exposure treatment. Finally, PFOS had no significant effects on P or F1 egg production and survival or whole-body vitellogenin levels in P or F1 male fish. The present investigation indicated a threshold for ecologically relevant adverse effects in zebrafish at 119 μg/L (standard deviation [S.D.] 23 μg/L, n = 10) for survival and 87 μg/L (S.D. 48 μg/L, n = 19) for all statistically significant negative effects observed. Importantly, males had significantly increased PFOS accumulation and bioconcentration factors versus females in both P and F1 generations. PFOS transfer to eggs was not a depuration pathway. Finally, a toxicokinetic model was developed to reliably predict PFOS whole-body burdens.

9 Feb 2024

Overview of Microscale Analytical Methods for the Quantitative Detection of Bioaccumulative Contaminants in Small Tissue Masses

Abstract: For many bioaccumulation studies, generation of large sample masses of exposed organisms is challenging or even prohibitive. Therefore, the use of smaller sample masses for analysis without compromising data quality or quantitative level achieved is desirable. To this end, a variety of microanalytical procedures have been developed that used 1 g or less of tissue to address specific experimental challenges. However, these methods have not been systematically evaluated or published. The present work evaluates the current state of the microanalytical methods reported and identifies additional needs that would benefit US Army Corps of Engineers (USACE) research and navigation dredging programs. Discussions with commercial laboratories revealed that they typically do not accept small sample masses and require individual sample masses ranging from 10 to 20 g wet weight of tissue per analysis. If they do analyze a small mass sample, they routinely do not modify their standard process, resulting in detection and reporting limits orders of magnitude higher; therefore, essentially useless nondetect data are generated for regulatory decisions. To address the lack of commercial availability of microanalytical methods, we recommend pursuing method development and subsequent validation of microscale extraction and analysis of a variety of common contaminant compounds in tissue matrices.

11 Aug 2021

Preparative, Extraction, and Analytical Methods for Simultaneous Determination of Legacy and Insensitive Munition (IM) Constituents in Aqueous, Soil or Sediment, and Tissue Matrices

Abstract: No standard method exists for determining levels of insensitive munition (IM) compounds in environmental matrices. This project resulted in new methods of extraction, analytical separation and quantitation of 17 legacy and 7 IM compounds, daughter products of IM, and other munition compounds absent from USEPA Method 8330B. Extraction methods were developed for aqueous (direct-injection and solid-phase extraction [SPE]), soil, sediment, and tissue samples using laboratory-spiked samples. Aqueous methods were tested on 5 water sources, with 23 of 24 compounds recovered within DoD QSM Ver5.2 limits. New solvent extraction (SE) methods enabled recovery of all 24 compounds from 6 soils within QSM limits, and a majority of the 24 compounds were recovered at acceptable levels from 4 tissues types. A modified chromatographic treatment method removed analytical interferences from tissue extracts. Two orthogonal high-performance liquid chromatography-ultraviolet (HPLC-UV) separation methods, along with an HPLC–mass spectrometric (HPLC-MS) method, were developed. Implementing these new methods should reduce labor and supply costs by approximately 50%, requiring a single extraction and sample preparation, and 2 analyses rather than 4. These new methods will support environmental monitoring of IM and facilitate execution of risk-related studies to determine long-term effects of IM compounds.