9 Apr 2026

Effects of Exopolysaccharides from Rhizobium tropici on Transformation and Aggregate Sizes of Iron Oxides

Abstract: Iron oxide transformations in soil significantly impact nutrient availability and plant health. This study investigated the interaction between exopolysaccharides (EPS), produced by Rhizobium tropici, and iron oxide (Fe3O4), focusing on their impact on the transformation, particle size, and zeta potential of iron oxides. The characterization of the EPS-iron oxide composites was carried out using X-ray Powder Diffraction (XRD), Fourier Trans-form Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM)/Energy Dispersive X-ray Analysis (EDX). The EPS adsorption kinetics revealed chemisorption and diffusion as controlling processes for EPS adsorption on Fe3O4, while isotherm data with releasing proton indicated possible ion exchange and heterogeneous layered adsorption. Desorption studies suggested the high stability of EPS-iron complexes. Notably, EPS significantly increased the aggregate size of EPS-iron complexes at low EPS/iron oxide molar ratios but shrank the aggregate size at higher ratios. Additionally, EPS complexation resulted in a shift in the zeta potential towards more negative surface functionality. Functional groups within EPS, specifically –COOH, –OH and –NH played a crucial role in the interaction of EPS with iron oxides. The study concluded that EPS coating prevented the transformation of Fe3O4 into other iron oxide forms like β-FeOOH, α-Fe2O3, and γ-Fe2O3, elucidating the significant role of EPS in soil mineral processes.

22 Jan 2026

Exopolysaccharides from Rhizobium Tropici Modified the Surface Characteristics of a Mississippi River Levee Soil Clay and its Bulk Soil Properties

Abstract: Global climate change has led to the increased frequency of extreme flooding events and heightened the vulnerability of river levees to flood related damage. One promising approach to enhancing the sustainability of levee stabilization is the use of eco-friendly, biologically produced soil additives as alternatives to conventional materials for erosion control. This study investigates the effects of exopolysaccharides (EPS) produced by Rhizobium tropici on the physical and engineering properties of clayey soil from a Mississippi River levee. Specifically, the study examines how EPS affects particle size, surface charge, surface area, and key bulk soil properties, including Atterberg limits, compaction behavior, and hydraulic conductivity. Soil samples were collected from a levee embankment located in south of Vidalia LA, an area historically prone to slough slides due to highly plastic nature of its floodplain clay soils. X-ray Power Diffraction was used to characterize the mineralogy of soil clay, EPS and EPS-clay composites. Particle size distribution and Zeta potentials measurements were performed on EPS and EPS-amended clays. Engineering test included Atterberg limit determinations (liquid and plastic limits) and standard compaction tests. The addition of EPS significantly increased the aggregate particle sizes of the levee clay through formation of EPS-clay composites. A strong correlation was observed between mean particle sizes and zeta potential in the composites. EPS also increased the liquid limit and plasticity of the soil while significantly reducing its hydraulic conductivity. Overall, EPS-amended soil demonstrated improved resistance to seepage and erosion, indicating that EPS has the potential to enhance levee soil stability and contribute to more sustainable flood control infrastructure.