5 Mar 2024

Environmental DNA Sampling for At-Risk and Invasive Species Management on Military Ranges: Guidelines and Protocols for Installation Biologists and Land Managers

Abstract: Environmental DNA (eDNA) analysis, or the detection of trace DNA shed by organisms into their environment, has the potential to transform Army capabilities for threatened and endangered species (TES) and invasive species management by providing a rapid, noninvasive, and cost-effective option for monitoring wildlife. Despite these benefits, eDNA analysis is underutilized on military installations as limited access to guidance materials, protocols, training opportunities, and support from eDNA scientists makes it difficult for installation biologists and military land managers to design and execute eDNA surveys, let alone identify management questions that may benefit from eDNA monitoring. Therefore, the aim of this resource is to increase awareness of the benefits and limitations of eDNA monitoring and provide eDNA study design guidelines and field sampling protocols for nonexperts to make this tool more accessible to installation biologists and land managers and help facilitate the adoption of eDNA-based approaches for wildlife management on military ranges.