12 Mar 2025

Reading the Ground: Understanding the Response of Bioelectric Microbes to Anthropogenic Compounds in Soil Based Terrestrial Microbial Fuel Cells

Abstract: Electrogenic bacteria produce power in soil based terrestrial microbial fuel cells (tMFCs) by growing on electrodes and transferring electrons released from the breakdown of substrates. The direction and magnitude of voltage production is hypothesized to be dependent on the available substrates. A sensor technology was developed for compounds indicative of anthropological activity by exposing tMFCs to gasoline, petroleum, 2,4-dinitrotoluene, fertilizer, and urea. A machine learning classifier was trained to identify compounds based on the voltage patterns. After 5 to 10 days, the mean voltage stabilized (+/- 0.5 mV). After the entire incubation, voltage ranged from -59.1 mV to 631.8 mV, with the tMFCs containing urea and gasoline producing the highest (624 mV) and lowest (-9 mV) average voltage, respectively. The machine learning algorithm effectively discerned between gasoline, urea, and fertilizer with greater than 94% accuracy, demonstrating that this technology could be successfully operated as an environmental sensor for change detection.

29 Dec 2022

Evaluating a Multi-Panel Air Cathode Through Electrochemical and Biotic Tests

Abstract: To scale up microbial fuel cells (MFCs), larger cathodes need to be developed that can use air directly, rather than dissolved oxygen, and have good electrochemical performance. A new type of cathode design was examined here that uses a “window-pane” approach with fifteen smaller cathodes welded to a single conductive metal sheet to maintain good electrical conductivity across the cathode with an increase in total area. Abiotic electrochemical tests were conducted to evaluate the impact of the cathode size (exposed areas of 7 cm², 33 cm², and 6200 cm²) on performance for all cathodes having the same active catalyst material. Increasing the size of the exposed area of the electrodes to the electrolyte from 7 cm² to 33 cm² (a single cathode panel) decreased the cathode potential by 5%, and a further increase in size to 6200 cm² using the multi-panel cathode reduced the electrode potential by 55% (at 0.6 A m⁻²), in a 50 mM phosphate buffer solution (PBS). In 85 L MFC tests with the largest cathode using wastewater as a fuel, the maximum power density based on polarization data was 0.083 ± 0.006Wm⁻² using 22 brush anodes to fully cover the cathode, and 0.061 ± 0.003Wm⁻² with 8 brush anodes (40% of cathode projected area) compared to 0.304 ± 0.009Wm⁻² obtained in the 28 mL MFC. Recovering power from large MFCs will therefore be challenging, but several approaches identified in this study can be pursued to maintain performance when increasing the size of the electrodes.