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Tag: Groundwater--Purification
  • Bioaugmentation for Enhanced Mitigation of Explosives in Surface Soil

    Abstract: Residual munition constituents (MCs) generated from live-fire training exercises persist in soil and can migrate to groundwater, surface waters, and off-range locations. Techniques to mitigate this potential migration are needed. Since the MC hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) can be biodegraded, soil inoculation with RDX-degrading bacteria (i.e., bioaugmentation) was investigated as a means to reduce the migration potential of RDX. Metagenomic studies using contaminated soils have suggested that a greater diversity of bacteria are capable of RDX biodegradation. However, these bacteria remain uncultivated and are potentially a source of novel enzymes and pathways for RDX biodegradation. In situ soil cultivation of a novel soil array was used to isolate the uncultivated bacteria that had been inferred to degrade RDX. Approximately 10.5% of the bacteria isolated from the soil arrays degraded RDX by the aerobic denitration pathway. Of these, 26.5% were possibly novel species of RDX-degrading bacteria, based on 16S rRNA sequence similarity. Both cell encapsulation in hydrogels and coating cells onto granules of polymeric carbon sources were investigated as carrier/delivery approaches for soil inoculation. However, neither of these approaches could confirm that the observed RDX degradation was by the inoculated bacteria.
  • Biopolymer Production in the Aquifer of a Groundwater Pump-and-Treat System

    Abstract: To establish the cause of filter clogging in the groundwater treatment system at Kirtland Air Force Base and assist in possible mitigation approaches.