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Design and Validation of Universal and Taxon-Specific 16S rRNA qPCR Primers for Detection of Freshwater Harmful Algal Bloom-Forming Cyanobacteria

US Army Engineer Research and Development Center
Published Sept. 22, 2025
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Vicksburg, Miss. --

Link : https://dx.doi.org/10.21079/11681/49953

Report Number: ERDC/EL MP-25-20

Title: Design and Validation of Universal and Taxon-Specific 16S rRNA qPCR Primers for Detection of Freshwater Harmful Algal Bloom-Forming Cyanobacteria

By Ping Gong, Anna K. Antrim, Alyxandra S. Cicerrella, Seung Ho Chung, Xiao Luo, Natalie D. Barker, Emily Cooley, and Qing Ji

Approved for Public Release; Distribution is Unlimited

September 2025

25 pages / 7.05 MB


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ERDC Library
601-634-2355
erdclibrary@chat.libraryh3lp.com

Release no. 25-235

Field validation
Genus/clade-specific primers
Nanopore sequencing
Performance evaluation metrics
Quantitative polymerase chain reaction (qPCR)
16S ribosomal RNA (rRNA)
Toxin-producing cyanobacteria

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