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Tag: Silver Carp
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  • Occurrence of Silver, Bighead, and Black Carp in Waters Managed by the US Army Corps of Engineers

    Abstract: This technical report (TR) documents the distribution of Invasive Carp - Silver Carp (Hypophthalmichthys molitrix), Bighead Carp (Hypophthalmichthys nobilis), and Black Carp (Mylopharyngodon piceus) in US Army Corps of Engineers (USACE) managed waters. Invasive carp were introduced into the US nearly half a century ago. As they spread across the US, they compete with and consume native species and Silver Carp jumping behavior reduces recreational use. The USACE is responsible for management of waterbodies at more than 440 projects. Information on these projects including the presence of Invasive Carp is reported in the USACE Operations and Maintenance Business Information Link (OMBIL) database. To supplement the Invasive Carp information from OMBIL, 47 online ichthyology collection and observation databases were searched; 18 had information on Invasive Carp locations. Combining the collection, observation and OMBIL reporting information, 82 USACE projects in 19 districts in 6 of 8 divisions have Silver/Bighead Carp and 19 projects in 9 districts and 3 divisions have Black Carp. Understanding the distribution of Invasive Carp is important to enable managers to be pro-active: planning control efforts, posting informational signs, instituting live bait restrictions, and thus reducing the chances of species introduction or limiting species impact.
  • Variation in Inhibitor Effects on qPCR Assays and implications for eDNA Surveys

    Abstract: Aquatic environmental DNA (eDNA) surveys are sometimes impacted by polymerase chain reaction (PCR) inhibitors. We tested varying concentrations of different inhibitors (humic, phytic, and tannic acids; crude leaf extracts) for impacts on quantitative PCR (qPCR) assays designed for eDNA surveys of bighead and silver carp (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix). We also tested for inhibition by high concentrations of exogenous DNA, hypothesizing that DNA from increasingly closely related species would be increasingly inhibitory. All tested inhibitors impacted qPCR, though only at very high concentrations — likely a function, in part, of having used an inhibitor-resistant qPCR solution. Closer phylogenetic relatedness resulted in inhibition at lower exogenous DNA concentrations, but not at relatively close phylogenetic scales. Inhibition was also influenced by the qPCR reporter dye used. Importantly, different qPCR assays responded differently to the same inhibitor concentrations. Implications of these results are that the inclusion of more than one assay for the same target taxa in an eDNA survey may be an important countermeasure against false negatives and that internal positive controls may not, in the absence of efforts to maximize inhibition compatibility, provide useful information about the inhibition of an eDNA assay.