Abstract: This report assesses potential impacts to aquatic resources resulting from proposed navigation channel expansion activities within Mobile Bay, Alabama. This work was conducted for the U.S. Army Corps of Engineers (USACE) Mobile District, to support development of a supplemental Environmental Impact Statement. Changes in water quality and hydrodynamics were evaluated for potential impacts to benthic macroinvertebrates, wetlands, submerged aquatic vegetation, oysters, and fish. The assessment includes extensive characterization of baseline conditions, evaluation of estimated post-project conditions related to aquatic resource habitat (e.g., changes in salinity, dissolved oxygen). An analysis of potential impacts related to a 0.5-m sea level rise scenario were also evaluated. Results suggest that no substantial impacts in aquatic resources within the study area are anticipated due to project implementation, as the area of greatest potential changes to environmental conditions are already adapted to natural shifts in salinity (and other factors), and to conditions resulting from the existing navigation channel. Although sea level rise has the potential to alter aquatic resource habitats with Mobile Bay, additional impacts related to project implementation remain negligible under the 0.5-m sea level rise scenario.